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(a) Comparison of relative abundances and believed absolute abundances of dominant bacterial people in 114 fecal samples. The best panel shows relative abundances based upon 16S amplicon sequencing and the decreased panels exhibits the approximated complete abundances calculated by multiplying the relative abundances with whole bacterial load, i.e. qPCR-based estimate of copies of 16S gene per one g of feces. (b) Correlation among the qPCR abundances (16S rRNA gene copies for every g feces) as well as estimated complete abundances of four taxa symbolizing species, genus, household and phylum degrees. The dashed line exhibits the anticipated 1:one correspondence.
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Importantly, qPCR-based mostly quantitative microbiome profiling enjoys the following conceptual and simple Advantages in excess of other approaches:
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